This includes supplemental figures and tables for Gang et al., 2025 "Generation and validation of an aryl hydrocarbon receptor knockout human embryonic stem cell line"
To verify whether the CcpA gene was successfully deleted, we designed the primer for PCR. The primer was designed across the upstream and downstream sequences of the CcpA gene, with a size of about 18
Protein lysates were collected from WT or Xrn1 ko A549 cells and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or b
