MicroRNA profile of tissue and discharge samples from cervical precancerous lesions

Cervical tissue and discharge samples were collected from patients with high-grade squamous intraepithelial lesion (HSIL) and adenocarcinoma in situ (AIS). Normal tissue samples were also collected for miRNA expression comparison. The samples were then sent for small RNA sequencing to obtain the miRNA counts for the miRNA profiles of the samples. Overall design: A total of 49 cervical tissue samples and 23 discharge samples were collected from 27 patients. The samples were grouped as HSIL (40 tissue and 19 discharge samples), AIS (4 tissue and 4 discharge samples), and normal (5 tissue samples). The samples were then sent for small RNA sequencing to obtain the miRNA counts for the miRNA profiles of each sample.