Supporting data for "Hi-C chromosome conformation capture sequencing of avian genomes using the BGISEQ-500 platform"

Hi-C experiments couple DNA-DNA proximity with next-generation sequencing to yield an unbiased description of genome-wide interactions. Previous methods describing Hi-C experiments have focused on the industry-standard, Illumina sequencing. With new next-generation sequencing platforms such as BGISEQ-500 becoming more widely available, protocol adaptations to fit platform-specific requirements are useful to give increased choice to researchers who routinely generate sequencing data. We describe an in-situ Hi-C protocol adapted to be compatible with the the BGISEQ-500 high-throughput sequencing platform. Using zebra finch (Taeniopygia guttata) as a biological sample, we demonstrate how Hi-C libraries can be constructed to generate informative data using the BGISEQ-500 platform, following circularization and DNA nanoball generation. Our protocol is a modification of an Illumina-compatible method, based around blunt-end ligations in library construction, using un-barcoded, distally overhanging double-stranded adapters, followed by amplification using indexed primers. The resulting libraries are ready for circularization and subsequent sequencing on the BGISEQ series of platforms, and yield data similar to what can be expected using Illumina-compatible approaches. Our straightforward modification to an Illumina-compatible in-situ Hi-C protocol enables data generation on the BGISEQ series of platforms, thus expanding the options available for researchers who wish to utilize the powerful Hi-C techniques in their research.Hi-C实验将DNA-DNA邻近互作与下一代测序（next-generation sequencing）相结合，可无偏地获取全基因组范围内的染色质相互作用信息。此前针对Hi-C实验的分析与建库方法多聚焦于行业主流的Illumina测序平台。随着BGISEQ-500等新型下一代测序平台的日益普及，开发适配不同平台专属要求的实验方案，可为常规开展测序工作的研究者提供更多选择空间。本研究报道了一套适配BGISEQ-500高通量测序平台的原位Hi-C实验方案。本研究以斑胸草雀（Taeniopygia guttata）为实验样本，展示了在完成环化反应与DNA纳米球（DNA nanoball）制备后，如何利用BGISEQ-500平台构建可产出有效测序数据的Hi-C文库。该方案基于适配Illumina平台的经典方法改造而成，文库构建以平末端连接为核心步骤，使用不带条形码的远端悬垂双链接头，随后通过带索引引物完成扩增反应。所构建的文库可直接用于BGISEQ系列平台的环化反应与后续测序，其产出的数据质量与基于Illumina兼容方案的预期结果相当。本研究仅对适配Illumina平台的原位Hi-C实验方案进行了简易改造，即可实现在BGISEQ系列平台上的测序数据产出，从而为希望将高效Hi-C技术应用于自身研究的研究者拓展了实验选择范围。