Distribution of radioactivity in different fractions after feeding an antisense (AS213), a control (Ctrl3) and a sense (S101) root clones with [U-14C]Suc.

Following incubation with the radioactive tracer, radioactive CO2 trapped in KOH was counted. Roots were extracted in 80% (v/v) ethanol and the extract fractionated into neutral, acidic and basic fractions. Aliquots of each fraction were counted. The radioactivity in the insoluble fraction represents the ethanol insoluble residue. Data are mean ± SE of 3 to 4 labeling experiments.