Identification of renal resident macrophages across species [10X]

We use single cell RNA sequencing to identify an evolutionarily conserved gene expression signature in resident macrophages. Based on our single cell data, we show the presence of a core gene expression signature in a cluster of innate immune cells in mouse, rat, pig, and human kidney tissue. Further, using this gene expression signature, we identify novel candidate markers for resident macrophages that span all 4 species tested. Overall design: We performed 10x genomics and fluidigm C1 single cell sequencing on innate immune cells isolated from the kidney of mouse, rat, pig, and human kidney tissue.