Total RNA-Seq of testis and ovaries of conventional raised (convR) and Germ-free (GF) female mice under ad libitum feeding regimen.

Gut microbiota and the circadian clock are both key regulators of the metabolic processes. Although recent evidence points to the impact of the circadian clock on microbiota, gut microbiota effect on diurnal host gene expression remains elusive. A transcriptome analysis of germ-free mice reveals subtle changes in circadian clock gene expression. However, a lack of microbiome leads to liver feminization and alters the expression of male-specific genes involved in lipid metabolism and xenobiotic detoxification associated with sustained activation of the Growth Hormone pathway. These results emphasize the mutual interaction of gut microbiota and its host even on unexpected functions. Total RNA-Seq of testis and ovaries of conventional raised (convR) and Germ-free (GF) female mice under ad libitum feeding regime.肠道菌群（gut microbiota）与生物钟（circadian clock）均为代谢过程的关键调控因子。尽管已有研究证实生物钟对肠道菌群具有调控作用，但肠道菌群对宿主昼夜节律基因表达的影响仍未明确。针对无菌小鼠（germ-free mice）的转录组分析（transcriptome analysis）显示，其生物钟基因表达存在细微改变。然而，无菌状态（即缺乏微生物组）会引发肝脏雌性化（liver feminization），并改变与脂质代谢及外源物解毒（xenobiotic detoxification）相关的雄性特异性基因的表达，该过程与生长激素通路（Growth Hormone pathway）的持续激活密切相关。上述研究结果凸显了肠道菌群与宿主之间的相互调控关系，甚至涉及此前未被预期的生物学功能。本数据集涵盖常规饲养（conventional raised, convR）与无菌（GF）雌性小鼠在自由进食模式（ad libitum feeding regime）下的睾丸与卵巢总RNA测序（Total RNA-Seq）数据。