Data sets for bacteriophage induction and effect of chitosan

(Figure 1A): Induction of TOX:GFP strain by ciprofloxacin and chitosan effectThe induction of TOX:GFP, an E. coli strain carrying a lysogneic 933W mutant bacteriophage in which the stx operon was replaced by a gene encoding GFP, and the effect of chitosan in vitro are shown below. The strain was was induced with ciprofloxacin and the optical density was measured at 600 nm over time (0, 2, 4, 6 hours).
(Figure 1B): Bacteriophage titersBacteriophage titers at different conditions are shown over time (hours). Bacteriophage titers were analyzed at 0, 2, 4 and 6 hours post-induction. Chitosan was added at 2 or 4 h post-induction.
(Figure 3B): GFP expression in vivo by IVISGFP expression in vivo in mice infected with C600TOX:GFP was measured using In Vivo Imaging System (IVIS). Radiant efficiency in organs of different groups is shown. Four animals per group were analyzed and the fluorescence intensity was quantified using Living Imaging 4.3.1 in Calipter Life Sciences. Each number is an individual mouse. N=5 per group.

图1A：通过环丙沙星和壳聚糖诱导TOX:GFP菌株及体外壳聚糖效应
下文展示了携带溶原性933W突变噬菌体的E. coli菌株TOX:GFP的诱导，其中stx操纵子被编码GFP的基因所替代，以及壳聚糖在体外的影响。该菌株通过环丙沙星诱导，并在0、2、4、6小时的时间点测量了600 nm处的光学密度。
图1B：噬菌体滴度
在不同条件下，噬菌体滴度随时间的变化情况展示如下（小时）。噬菌体滴度在诱导后0、2、4和6小时进行分析。壳聚糖在诱导后2或4小时加入。
图3B：感染C600TOX:GFP小鼠体内的GFP表达
利用活体成像系统（IVIS）测量感染C600TOX:GFP小鼠的体内GFP表达。不同组别器官的辐射效率展示如下。每组分析4只动物，并使用Calipter Life Sciences的Living Imaging 4.3.1软件量化荧光强度。每个数字代表一只单独的小鼠。每组样本数量为N=5。