Nexus-formatted text file of ISSR band presence/absence for 75 loci in Corallorhiza bentleyi

Mycoheterotrophic plants derive most or all carbon and nutrients from fungal partners and represent poorly understood components of forest biodiversity. Many are rare or endangered yet can be ecological indicators of forest ecosystem function due to their often highly specific fungal host requirements. One such species is the IUCN red-listed (‘vulnerable’), fully mycoheterotrophic orchid, Corallorhiza bentleyi. This recently described species is among the rarest plants in Appalachia, known from five counties in Virginia and West Virginia, USA. The species has a restricted range, small population size, and is self-pollinating. Here, an integrative approach was taken in conservation genetic assessment of C. bentleyi using floral morphometrics, simple-sequence repeats, and fungal host DNA to characterize variation within and among sampling localities. Morphology reveals some differentiation among individuals from six sampling localities. Surprisingly, most genetic variation is found within...菌异养植物（mycoheterotrophic plants）可从真菌共生伙伴处获取绝大部分乃至全部碳源与营养物质，是森林生物多样性中研究程度较低的类群。该类群多数物种珍稀或濒危，且因通常具有高度专一的真菌宿主偏好，可作为森林生态系统功能的生态指示物。其中典型代表之一是被国际自然保护联盟（IUCN）红色名录列为‘易危（vulnerable）’的全菌异养型兰科植物——本特利珊瑚兰（Corallorhiza bentleyi）。这一近年新定名的物种是阿巴拉契亚地区最为珍稀的植物之一，目前仅记录于美国弗吉尼亚州与西弗吉尼亚州的5个县域。该物种分布范围狭窄、种群规模微小，且为自花授粉植物。本研究采用整合性研究策略，通过花部形态测量学、简单序列重复（simple-sequence repeats）标记及真菌宿主DNA分析，对本特利珊瑚兰开展保护遗传学评估，以解析采样点内及采样点间的遗传变异模式。形态学分析显示，来自6个采样点的个体间存在一定程度的分化。令人意外的是，绝大多数遗传变异存在于