Employed three different RNAi methodologies in wild type N2 strain of <i>C.elegans</i> for dumpy phenotype.

Method I, RNAi clone of dpy-9 co-incuabted with RNAi enhancers (eri-1 and lin-35); Method II, Pre-incubation with eri-1 RNAi clone followed by incubation with dpy-9 RNAi clone, co-incubation with eri-1 and dpy-9 RNAi clones and co-incubation with dpy-9, eri-1 and lin-35 RNAi clones; Method III, Pre-incubation with dpy-9 RNAi clone followed by incubation with eri-1, co-incubation with dpy-9 and eri-1 RNAi clones and co-incubation with dpy-9, eri-1 and lin-35 RNAi clones. dpy, dumpy; WT, wild-type. +,weak phenotype; ++, medium phenotype; +++, strong phenotype.