AmrZ affinity for amrZ1 binding site mutants.

aThe two AmrZ binding sites on the wild type amrZ1 sequence are represented by the underlined nucleotides. Mutations to the wild type amrZ1 binding site are notated by the bolded nucleotides in each mutant sequence.bThe Kd was calculated by fitting the hyperbolic equation for a single ligand binding model with saturation (eq 2) to the data in Figure S2, which were averaged from four independent experiments.cFold over (wild type) WT amrZ1 is defined by (Kd of sample)/(Kd of wild type) for each sample.