Comparison between GT and hoxH mutant strains of cyanobacteria

HoxH is a subunit of hydrogenase in a unicelluar cyanobacterium Synechocystis sp. PCC6803. We constructed hoxH mutant strain, which the mRNA levels of hoxH reduced to 50% of the wild-type. Total RNAs from three independent wilde-type (GT) and three independent hoxH mutant were labeled by Cy3 followed by hybridyzation on a microarry chip generated by Agilent Technologies (Design ID 036279). Microarray is designed by Prof. Tomohisa Hasunuma, Kobe University. CITATION: Metabolomics-based analysis revealing the alteration of primary carbon metabolism by the genetic manipulation of a hydrogenase HoxH in Synechocystis sp. PCC 6803 Iijima H, Shirai T, Okamoto M, Pinto F, Tamagnini P, Hasunuma T, Kondo A, Hirai MY, *Osanai T (2016) Algal Res. 18: 305-313, doi.org/10.1016/j.algal.2016.06.026