snRNA-seq of mouse (Mus musculus) adult testis

Single-nucleus RNA sequencing (snRNA-seq) was used to profile the transcriptome of 5,264 nuclei in mouse adult testis. This dataset includes two samples from two different individuals. This dataset is part of a larger evolutionary study of adult testis at the single-nucleus level (97,521 single-nuclei in total) across mammals including 10 representatives of the three main mammalian lineages: human, chimpanzee, bonobo, gorilla, gibbon, rhesus macaque, marmoset, mouse (placental mammals); grey short-tailed opossum (marsupials); and platypus (egg-laying monotremes). Corresponding data were generated for a bird (red junglefowl, the progenitor of domestic chicken), to be used as an evolutionary outgroup.本研究采用单细胞核RNA测序（single-nucleus RNA sequencing, snRNA-seq）技术，对成年小鼠睾丸的5264个细胞核的转录组进行了图谱绘制。该数据集包含来自2名不同个体的2份样本，属于一项更大规模的成年睾丸单细胞核水平进化研究的组成部分。该项整体研究共计涵盖97521个单细胞核，涉及哺乳类动物三大主要演化支系的10个代表物种：人类、黑猩猩、倭黑猩猩、大猩猩、长臂猿、恒河猴、普通狨、小鼠（胎盘类哺乳动物）；灰短尾负鼠（有袋类哺乳动物）；以及鸭嘴兽（产卵单孔目哺乳动物）。此外，该研究还为一种鸟类——红原鸡（家鸡的祖先）生成了对应测序数据，以作为进化分析的外类群。