Dimethyl fumarate treated TC-1 cells

TC-1 cells were treated with 50 µM DMF or the same dose of DMSO for 24 hrs. RNA was extracted from these samples and sent to BGI (China, Shenzhen) for sequencing. The cDNA library was constructed using the BGISEQ-500 platform. After obtaining clean reads, they were aligned to the reference genome sequence (GCF_000001635.26_GRCm38.p6) using HISATs. mRNA enrichment and purification: Oligo dT Selection to enrich the mRNA (For total RNA extracted from human whole blood, globin mRNA are depleted.Sequencing data is called raw reads or raw data, and quality control (QC) is then performed on the raw reads to determine whether the sequencing data is suitable for subsequent analysis. After quality control, the filtered clean reads were aligned to the reference sequence.  After the alignment, the statistics of the mapping rate and the distribution of reads on the reference sequence are used to determine whether the alignment result passes the second QC of alignment. If it passes, we perform gene quantification analysis and other analysis based on gene expression (principal component, correlation, differential gene screening, etc.), and perform significant enrichment analysis of GO function on differentially expressed genes among the screened samples, significance enrichment analysis of pathway, clustering, protein interaction networks, and transcription factors, and more in-depth mining analysis. Processed data files are tab-delimited text files including raw counts for each sample: Control1_1.fq, Control1_2.fq; Control2_1.fq, Control2_2.fq; Contro3_1.fq, Contro3_2.fq; DMF1_1.fq, DMF1_2.fq; DMF2_1.fq, DMF2_2.fq; DMF3_1.fq, DMF3_2.fq.