Quantitative phytoplankton profiles for Pensacola Transect Station P1 collected during the R/V Bellows cruise of DeSoto Canyon, May 31, 2014

This dataset reports phytoplankton species abundance data for samples collected from the R/V Bellows during the May 31-June 1, 2014 cruise. Samples were taken at Pensacola Transect Station P1 (30.25o N, 87.25o W) in the Florida Panhandle Bight at the head of DeSoto Canyon. One-liter samples were collected from the surface and a depth 17 meters using Niskin bottles. Up to one liter of each sample was filtered onto a 0.45 micrometer nitrocellulose filter and air-dried. A portion of each filter was mounted on an aluminum stub, sputter-coated, and examined with a JEOL JSM-6480 LV scanning electron microscope. Diatoms, coccolithophores, and armored dinoflagellates were identified to the lowest possible taxonomic category. The number of cells of each taxon in a field of view at a magnification of 1000x was recorded, with up to 700 fields-of-view examined, depending on the density of phytoplankton in the sample. The dataset includes the depth, the volume filtered, the number of cells of each taxon counted, and the calculated abundance of each taxon in cells per liter. Micrographs taken during the analysis are included.本数据集记录了2014年5月31日至6月1日期间，搭载于贝尔洛斯号研究船（R/V Bellows）所采集样本的浮游植物（phytoplankton）物种丰度数据。样本采集自德索托海谷顶端的佛罗里达狭长海岸湾彭萨科拉断面P1站位（北纬30.25°，西经87.25°）。采用尼斯金采水器（Niskin bottles）分别从表层海水及17米深度处采集1升体积的样本。每份样本最多取1升进行过滤，滤膜采用孔径0.45微米的硝酸纤维素滤膜（nitrocellulose filter），随后进行风干处理。取部分滤膜安装于铝样品台（aluminum stub）上，经溅射镀膜后，使用JEOL JSM-6480 LV型扫描电子显微镜进行观测。对硅藻（diatoms）、颗石藻（coccolithophores）及具壳甲藻（armored dinoflagellates）进行了尽可能精细的分类鉴定，直至最低分类阶元。在1000倍放大倍率下的每个视野中，记录各分类单元的细胞数量；依据样本中浮游植物的密度，最多可观测多达700个视野。本数据集包含采样深度、过滤体积、各分类单元的计数细胞数，以及以每升细胞数为单位计算得到的各分类单元丰度。分析过程中拍摄的显微照片亦包含于本数据集内。