Data, code, and output files associated with: Polarization-sensitive photoreceptors inform host-seeking in stable flies

This dataset is a supplement to the article "Polarization-sensitive photoreceptors inform host-seeking in stable flies". The article included a combination of anatomical, electrophysiological and behavioral investigations of vision in stable flies. The methods describing the generation of this data is fully described in the associated article. Article Abstract: "Insects use their polarization-sensitive photoreceptors in a variety of ecological contexts including host-foraging. Here, we investigated the effect of polarized light on host foraging by the blood-feeding stable fly, Stomoxys calcitrans, a pest of livestock. Electroretinogram recordings with chromatic adaptation demonstrated that the spectral sensitivity of stable flies resembles that of other calyptrate flies. Histological studies of the flies’ compound eye revealed differences in microvillar arrangement of pale and yellow ommatidial types, with the pale R7 and R8 photoreceptors having the greatest polarization sensitivity. In behavioural experiments, stable flies preferred to alight on horizontally polarized stimuli with a high degree of linear polarization. This preferential response disappeared when either ultraviolet (UV) or human-visible wavelengths were omitted from light stimuli. Removing specific wavelength bands further revealed that the combination of UV (330–400 nm) and blue (400–525 nm) wavelength bands was sufficient to enable polarized light discrimination by flies. These findings enhance our understanding of polarization vision and foraging behavior among hematophagous insects and should inform future trap designs." This dataset contains: ---"anatomy"--- Original and modified electron microscopy images of transverse sections of pale and yellow ommatidia taken at multiple points along their length. Along with a 3D slice model used to estimate off axis cutting distortions. ---"behavioral bioassays"--- Data files, R scripts, and output graphs for the behavioral bioassay experiments (Exp 1-11, see table S1) ---"electrophysiology"--- Raw recordings, R scripts, intermediate files, and output files relating to the electroretinogram recordings of female stable fly compound eyes under chromatic adaptation. ---"polarimetry"--- Images and output files associated with photo polarimetry of filter stack stimuli (see Table S1) ---"spectroscopy"--- Emission, transmission, and reflectance measurements, along with the R code used to generate Fig. S1本数据集为论文《偏振敏感感光细胞调控厩螫蝇的宿主搜寻行为》（Polarization-sensitive photoreceptors inform host-seeking in stable flies）的补充材料。该论文整合了厩螫蝇视觉系统的解剖学、电生理学与行为学研究，本数据集的生成方法已在相关论文中完整详述。 论文摘要： 昆虫的偏振敏感感光细胞可应用于多种生态场景，其中包括宿主觅食。本研究针对吸血性畜牧害虫厩螫蝇（Stomoxys calcitrans）的偏振光对其宿主搜寻行为的影响展开探究。通过色素适应条件下的视网膜电图（electroretinogram）记录发现，厩螫蝇的光谱敏感性与其他有瓣蝇类相似。对其复眼的组织学研究显示，浅色与黄色小眼的微绒毛排列存在差异，其中浅色R7与R8感光细胞的偏振敏感性最强。行为学实验结果表明，厩螫蝇更倾向于停落在高线性偏振水平的水平偏振刺激表面。当移除紫外（UV，ultraviolet）或人类可见光波段时，这一偏好性响应会消失；进一步移除特定波长波段后发现，仅需紫外（330~400 nm）与蓝（400~525 nm）波段的组合，即可让厩螫蝇实现偏振光辨别。本研究结果加深了我们对吸血昆虫偏振视觉与觅食行为的理解，可为未来的诱捕装置设计提供参考。 本数据集包含以下内容： ---"解剖学"--- 沿小眼纵轴多个位点采集的浅色与黄色小眼横切面的原始及修正电子显微镜图像，以及用于估算离轴切割畸变的三维切片模型。 ---"行为学生物测定"--- 行为学生物测定实验（实验1~11，详见补充表S1）相关的数据文件、R脚本及输出图表。 ---"电生理学"--- 色素适应条件下雌性厩螫蝇复眼视网膜电图记录的原始数据、R脚本、中间文件及输出文件。 ---"偏振测定"--- 与滤光片组刺激物偏振光度测定相关的图像及输出文件（详见表S1）。 ---"光谱学"--- 发射、透射及反射率测量数据，以及用于生成补充图S1的R代码。