LINGO Receptor Family Members Control Epithelial Wound Healing Pathways

Wound healing requires orchestration of cellular migration, adhesion molecule synthesis, and cell cycle regulation. However, the cellular receptors necessary for the repair of damaged tissue remain poorly understood. This study investigated whether extracellular transmembrane receptors in the leucine rich repeat and nogo interacting protein family (LINGO) were required for the recovery of airway epithelial cells from from scratch wound injury. CRISPR/Cas9-mediated deletion mutants for either LINGO1, LINGO2, or LINGO3 or their putative co-receptors, Tumor Necrosis Factor Receptor Superfamily, Member 19 (TROY) and Reticulon 4 Receptor (RTN4R) in the mouse airway epithelial cell line MLE-12 were used for mRNA sequencing. Cells for all lines were grown to a monolayer and subjected to a scratch with a pipette tip. Cells from each mutant line and the parental line were collected for sequencing after 24 hours post-scratch. In addition the parental line was sequenced under baseline un-scratch conditions. Overall design: 6 samples total, no replicates, one parental line un-scratched, one parental line 24 hours post-scratch, Lingo1, Lingo2, Lingo3, and TROY mutant lines 24 hours post-scratch