High-Throughput Metabolomics using 96-plex Isotope Tagging

Liquid chromatography–mass spectrometry (LC-MS)
based metabolomics
suffers from extended duty cycles and matrix-dependent quantitation.
Chemical tags with 96 unique masses are reported, which alleviate
the metabolomic workflow bottleneck and allow for absolute quantitation.
A metabolic screen for carboxylic acids was performed on mammalian
cells deprived of various nutrients and showed 24% RSD and analysis
of 288 samples in 2 h.