Raw Dataset (PFN1-PI-RPL)

There is little information about the molecular processes that make up the interaction between the endometrium and the developing embryo, which is essential for successful continuation of pregnancy. Despite extensive research, recurrent pregnancy loss (RPL) remains a mystery in half of cases. Profilin-1 (PFN1) is a dominant regulatory protein in the cytoskeletal network. The aim of this study was to investigate the role of serum PFN1 in patients with primary idiopathic RPL (PI-RPL). A prospective cohort study was conducted with non-pregnant PI-RPL patients and healthy controls. After blood collection and centrifugation, serum samples were stored under appropriate conditions to be analyzed simultaneously after completion of sample collection from all participants. Serum PFN1 levels were measured using an enzyme-linked immunosorbent assay and compared between groups. The study was completed with 38 and 36 participants in the PI-RPL and control groups, respectively. PFN1 levels were significantly lower in the PI-RPL group compared to the control group (20.8±13.0 vs. 42.0±22.3, p=0.023), while baseline characteristics and inflammatory status, as determined by blood count-derived inflammatory indices, did not differ. However, PFN1 values did not appear to have high diagnostic accuracy, as the area under the curve was 0.654. On the other hand, according to the estimated marginal means of PFN1 levels, a value of 16.7 ng/dL is 68% predictive of PI-RPL. Our results show an association between low PFN1 levels and an increased risk of PI-RPL. However, further studies with larger sample sizes are needed to validate a cut-off value and improve its relevance.