Quantification of protein level changes under elevated mitochondrial abasic site damage

The project aims to examine the protein level changes under induced mitochondrial abasic site lesions in HeLa cells using stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics. The data revealed significant changes in the expression level of several proteins. A decrease in mitochondrial ribosomal proteins (MRPS35, MRPL15, MRPL37, MRPL13, and MRPL4) and complex I subunits (NDUFA12 and NDUFA13) was observed, suggesting a suppression of mitochondrial protein synthesis and energy production under these conditions. In addition, the levels of ARF5 (ADP ribosylation factor 5), DNM1L (dynamin-1-like protein), and PRDX6 (peroxiredoxin 6) were upregulated. ARF5 is involved in protein trafficking, and DNM1L mediates mitochondrial and peroxisomal division.