Gene expression analysis of in vitro activated B cells from C57BL/6 and Ackr4-deficient mice

To further understand differential phenotypes between C57BL/6 and Ackr4-deficient B cells, we have employed whole genome microarray expression profiling as a discovery platform to identify genes which are differentially expressed in the two genotypes. Murine B cells from spleens of C57BL/6 and Ackr4-deficient mice were purified by negative magnetic activated cell sorting (MACS) and activated in vitro with 10 µg/mL polyclonal anti-IgM and10 µg/mL anti-CD40 (clone FGK4.5) antibodies for three days in culture (96well plate, 37°C, 5%CO2). On day three, living B cells were sorted and RNA was extracted. Differential gene expression of murine in vitro activated B cells from spleens of C57BL/6 and Ackr4-deficient mice was measured at day 3 of activation. Two independent experiments were performed with different donors for each experiment.