Raw dataset of seminal proteomic from four purebred dogs

Hypothesis: Does dogs exhibit different protein profile of seminal plasma and spermatozoa among breeds? What data shows: These data show the proteomic profile and its respectively gene ontology of seminal plasma and sperm cells of four purebred dogs (Golden Retriever n = 3, Bernese Mountain Dog n = 4, Great Dane n = 3, Maremmano-Abruzzese Sheepdog n = 3), with mean ages and standard deviation of 4,0 ± 1,0 years (Golden Retriever), 2,0 ± 1,0 years (Bernese Mountain Dog), 1,4 ± 0,5 years (Great Dane) and 4,0 ± 0,7 years (Maremmano-Abruzzese Sheepdog), kenneled at Sao Paulo State, Brazil. Besides How it was gathered: Entire second fraction and a portion of the third semen fraction were collected into a silicone funnel attached to a graduated plastic tube by manual stimulation of the penis in the presence of a teaser bitch, when possible. The semen was subjectively evaluated at the kennel, and only ejaculate within normal seminal parameters considered for dogs, according to Kustritz et al. (2007), were used in this study. Spermatozoa and seminal plasma were separated by centrifugation and prepared individually for proteomic analysis by ESI Q-Tof mass spectrometer. The gene ontology annotation of the proteins found within the samples was obtained using the UniprotKB website (www.uniprot.org), and considered the molecular function, biological process and cellular component categories. How the data can be interpreted: There are two folders dataset. The "Seminal plasma and sperm cell proteins" folder contain two folders, one with all seminal plasma proteins, and other folder with all sperm cell proteins, which have individual files named by breed for each dog (n=13). The “Gene ontology of seminal plasma and sperm cell proteins” contain three files: Table S1, Table S2, and Table S3. The file Table S1 contain all proteins found in seminal plasma of evaluated dogs and their respective gene ontology. The file Table S2 contain all proteins found in spermatozoa of all dogs evaluated and their respective gene ontology. The file Table S3 contain all common proteins found in seminal plasma and spermatozoa of evaluated dogs and their respective gene ontology. References: Kustritz R. The value of canine semen evaluation for practitioners. Theriogenology 2007;68(3):329-37.研究假设：不同品种犬的精浆（seminal plasma）与精子（spermatozoa）的蛋白质谱是否存在差异？ 数据集内容：本数据集呈现了4个纯种犬（金毛寻回犬 n=3、伯恩山犬 n=4、大丹犬 n=3、马雷马诺-阿布鲁泽塞牧羊犬 n=3）的蛋白质组谱（proteomic profile）及其对应基因本体（gene ontology）注释信息；各品种犬的平均年龄±标准差分别为：金毛寻回犬4.0±1.0岁、伯恩山犬2.0±1.0岁、大丹犬1.4±0.5岁、马雷马诺-阿布鲁泽塞牧羊犬4.0±0.7岁，所有受试犬均饲养于巴西圣保罗州。 数据采集方式：在可行条件下，借助诱情母犬，通过手动刺激公犬阴茎，将全部第二精液组分及部分第三精液组分收集至连接有刻度塑料试管的硅胶漏斗中。采集的精液在饲养场进行主观质量评估，仅采用符合Kustritz等人（2007年）制定的犬正常精液参数标准的射精样本用于本研究。通过离心分离精子与精浆，并分别制备样品，采用电喷雾电离四极杆飞行时间（ESI Q-Tof）质谱仪进行蛋白质组学分析。利用UniProtKB数据库（www.uniprot.org）对样本中鉴定出的蛋白质进行基因本体注释，注释涵盖分子功能、生物过程及细胞组分三大类别。 数据说明与文件结构：本数据集包含两个一级文件夹：其一为"Seminal plasma and sperm cell proteins"文件夹，内含两个子文件夹，分别存储所有受试犬的精浆蛋白质组数据与精子细胞蛋白质组数据，各子文件夹中包含按品种命名的单只受试犬数据文件（共13份）；其二为"Gene ontology of seminal plasma and sperm cell proteins"文件夹，内含三个文件：表S1、表S2与表S3。其中表S1收录受试犬精浆中鉴定出的全部蛋白质及其对应基因本体注释；表S2收录受试犬精子细胞中鉴定出的全部蛋白质及其对应基因本体注释；表S3收录受试犬精浆与精子细胞中共有的全部蛋白质及其对应基因本体注释。 参考文献：Kustritz R. 执业兽医犬精液评估的价值. 《兽产科学》(Theriogenology), 2007;68(3):329-37.