Formula used for preparation of the ointment.

BackgroundChronic wounds present a major health challenge, and traditional remedies are frequently employed in developing nations for wound treatment. This study sought to assess the safety and effectiveness of various solvent extracts from Cordia africana using excision and incision wound models in mice.MethodsThe dried, coarsely ground 500 g of plant leaves were separately macerated three times with 80% methanol, absolute methanol, chloroform, and aqueous water. Acute dermal toxicity was assessed by applying 2000 mg/kg of the 10% w/w of the solvent extracts. In both wound models, animals were randomly divided into four groups of five mice each. Group I received a simple ointment and served as the negative control. Group IV was treated with nitrofurazone (0.2%) ointment as the positive control. Groups II and III were treated with 5% w/w and 10% w/w ointments of the solvent extracts, respectively.ResultsThe acute dermal toxicity studies demonstrated that the plant leaf extracts are safe, as there were no signs of toxicity observed. In the excision wound model, the application of 5% and 10% ointments of the 80% methanol extract resulted in significant (p p p p ConclusionThe study revealed that Cordia africana leaf extracts possess significant wound-healing properties, supporting its traditional medicinal use. It was found that using less polar solvents is more effective in extracting components with enhanced wound-healing effects.