RNA-seq of LSC-like and Mature populations from human AML patient samples

Acute myeloid leukemia (AML) is a highly heterogeneous disease consisting of different cell states that drive therapy resistance. We aimed to transcriptionally characterize AML subpopulations from patients treated with standard chemotherapy or BCL-2 inhibitor Venetoclax. We used CD64 and CD11b expression to define Mature blasts and labelled the rest as Immature. We used GPR56 expression to further enrich for LSC-like cells within the Immature cells. We performed RNA sequencing on 169 FACS-sorted LSC-like (GPR56+) and 111 Mature (CD64+CD11b+) cells.