Mouse ureter: wild type vs miR-143/145 knock out

mRNA profiling of mouse ureters comparing wild-type ureter vs. ureters from mice having whole body deletion of miR-143 and miR-145 which results in abnormal ureter peristalsis and hydronephrosis. We used microarrays to detail the global program of gene expression in wild-type and miR-143/145-deficient ureters which revealed dysregulation of genes linked to smooth muscle morphology and function. Two condition experiment: wild type vs miR-143/145 KO; biological replicates: individual mice - 2 wild type, 2 mutant. One replicate per array.本数据集针对小鼠输尿管开展mRNA表达谱分析，对比野生型输尿管与全身敲除miR-143和miR-145的小鼠输尿管——该基因敲除会导致输尿管蠕动异常与肾积水。我们通过微阵列（microarray）技术全面解析野生型与miR-143/145缺陷型输尿管的全局基因表达模式，结果发现与平滑肌形态及功能相关的基因存在表达失调。本实验为双处理组对照设计：野生型组与miR-143/145基因敲除（KO）组；生物学重复采用单只小鼠样本，其中野生型、突变型各2例，每块阵列对应一个生物学重复样本。