Methods and data related to the publication " Safety and growth promoting potential of repeated administration of sodium salicylate to broilers "

This file is part of "Methods and data related to the publication " Safety and growth promoting potential of repeated administration of sodium salicylate to broilers "". The data presented here refer to the study of the inclusion of the non-steroidal anti-inflammatory sodium salicylate in the diet of male broilers of the Cobb 500 strain, during 42 days of life. The objective of this work was to evaluate the possible adverse effects of the prolonged consumption of this drug, as well as to evaluate the improvement in the zootechnical indices of these animals, such as increased weight gain and improved feed conversion. The data obtained were statistically evaluated using the SAS/STAT software program (SAS Institute, Cary, NC, USA) with the alpha value set to 0.05. Linear and quadratic polynomial contrasts were specified for dose-response modeling with correction for unequal spacing of SS doses, namely 0, 2.5, 5.0, and 10.0 mg/kg per day. Data subject with repeated measurements (BW, BWG, FI, FC) from the same individuals over a period of time were subjected to the linear mixed model, with treatment, time and the treatment x time interaction as fixed effects (available as supplemental material). The results were positive regarding possible adverse effects, showing that there was no toxicity to the animals at the doses used. However, there was no statistically significant improvement in broiler weight gain, but there was a non-statistical increase in the group treated with the highest dose. Because of this, further investigations must be carried out.本文件隶属于题为《重复施用水杨酸钠对肉鸡的安全性及促生长潜力》的已发表论文配套方法与数据集。本数据集所载试验针对科宝500品系雄性肉鸡展开，在其日粮中添加非甾体抗炎药水杨酸钠（sodium salicylate），试验周期覆盖肉鸡42日龄的全生长期。本研究的核心目标为评估长期摄入该药物可能引发的不良反应，同时探究该药物对肉鸡畜牧生产性能指标的改善效果，包括增重提升与饲料转化率优化。研究所得数据采用SAS/STAT统计软件（SAS研究所，美国北卡罗来纳州卡里市）开展统计学分析，设定显著性水平α为0.05。针对剂量-效应关系建模，采用线性与二次多项式对比法，并对水杨酸钠（SS）剂量的非均匀间距进行校正，所用剂量分别为0、2.5、5.0及10.0 mg/kg/日。针对同一受试个体在不同时间点的重复测量数据（BW（Body Weight，体重）、BWG（Body Weight Gain，体重增重）、FI（Feed Intake，采食量）、FC（Feed Conversion Ratio，饲料转化率）），采用线性混合模型进行分析，将处理组、时间以及处理×时间交互效应设为固定效应（相关内容见补充材料）。在潜在不良反应方面，试验结果呈正向结论：在所使用的剂量范围内，该药物未对肉鸡产生毒性作用。但在肉鸡增重方面，未观察到统计学意义上的显著改善；不过最高剂量处理组的肉鸡增重出现了未达统计学显著性的升高趋势。基于上述结果，仍需开展进一步的相关研究。