Transriptome profiling of mutant p53 skin deficient in AURORA-A KINASE

We performed next-generation sequencing of RNA to determine the transcriptional changes in hyperplastic mutant p53 skin after the epithelial deletion of the AURORA-A Kinase gene in mice. In this study skin RNA of four experimental groups were sequenced. Mice with the p53f, p53lslR172H, and KrasLSLG12D knock in alleles were crossed with the K14CreER(T) transgene to generate Loss-Of-Function (LOF) p53 mice (p53f/lslR172H;KraslslG12D;K14CreER) or GOF p53 mice (p53f/lslR172H;KraslslG12D;K14CreER). A floxed Aur-A allele was introduced to GOF or LOF p53 mice to generate Aur-Af/f; GOF or Aur-Af/f; LOF p53 mice. Tamoxifen was given to 8-10wk old mice in the diet for 14 d to induce recombination of floxed alleles. The mice were also co-treated with topical 12-O-tetradecanoylphorbol-13-acetate for 7d. Skin samples were harvested at the end of the study period.