10x Genomics VDJ seqeuncing of IOMAgl germinal center B cells

This experiment was performed to identify somatic hypermutations in germinal center B cells of a mouse expressing the inferred germline of IOMA BCR after and a 5 step sequential immunization regimen. This experiment was performed to identify somatic hypermutations in germinal center B cells of mouse HP3, homozyogous for the IghIOMAiGL and IgkIOMAiGL transgenes expressing the inferrred germline of IOMA after and a 5 step immunization regimen. The mice were immunized sequentially with mi3 nanoparticles. First primed with IGT2-mi3 and sequentially boosted with IGT1-mi3 (at week 5), 426c degly2 D279N-mi3 (week 10) , and twice with mosaic8-mi3 (week 15 and 20). HP3 was sacrified in week 23 and spleen and mesenteric lymph nodes were processed and stored in LN2. Then Germinal center B cells were sroted as live, singlet, lineage dump-, B220+, CD95+ CD38- lymphocytes.