Supporting File S1 - Soluble Human Leukocyte Antigen-G5 Activates Extracellular Signal-Regulated Protein Kinase Signaling and Stimulates Trophoblast Invasion

Figure S1 Effect of HLA-G5 on trophoblast proliferation. The proliferation rates of trophoblasts were not affected by HLA-G5 (0.1 or 1 µg/mL) treatments. However, at higher a concentration of HLA-G5 (10 µg/mL), the proliferation rates of JAr and JEG-3 cells were significantly reduced compared to the untreated control (N = 8, pFigure S2 Effect of HLA-G5 on trophoblast migration. The migration rates of JAr and JEG-3 trophoblasts were not affected by HLA-G5 (0.1 or 1 µg/mL) treatments (N = 8). The results are presented as the percentage of fluorescence intensity relative to the untreated group. Figure S3 Gelatin zymography of MMP2/MMP9 activity on HLA-G5 treated trophoblastic cells. In JAr cells, the activity of MMP9 was induced with HLA-G5 (1 µg/mL) treatment (pTable S1 Changes of uPA/MMPs expression and activity in trophoblast. In JAr cells, the expression of MMP2 increased significantly (pTable S2 ERK phosphorylation in trophoblast. The phosphorylated ERK was significantly induced after HLA-G5 treatment (p (DOC)