Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells.

Precision run-on sequencing (PRO-seq) of nascent RNA synthesis across the human genome. The PRO-seq data was generated from human K562 erythroleukemia cells that were either unconditioned (unCond) or preconditioned with multiple heat shock exposures (preCond). After two day recovery from the preconditioning, during which the cells underwent two mitotic divisions, the cells were additionally subjected to a single heat shock to measure nascent transcription in response to heat shock in unconditioned versus preconditioned cells. Overall design: Genome-wide nascent transcription profiles from human K562 cells that were either unconditioned or preconditioned with multiple heat shock exposures. The K562 cells' ability to mount transcriptional response to stress in unconditioned versus preconditioned cells was measured in the presence (scrambled sequence transfected) or absence (shHSF1) of the master transcriptional activator upon heat shock, HSF1.