Supplemental Material - Phytochemical extracts from red sorghum (Sorghum bicolor, (L.) Moench) leaves and stems improve nitrogen efficiency in dairy cows by modulating rumen microbiota

Supplemental Table 1. The ingredient and chemical composition of the TMR (% of DM). Supplemental Table 2. ANOVA results for the effects of extraction variables (ethanol concentration, time, repetitions) on yield, TPC, and TFC. Supplemental Table 3. Regression coefficients and ANOVA of regression parameters of the predicted response surface quadratic models. Supplemental Table 4. Comparison of predicted vs. experimental values for extraction yield, TPC, and TFC under optimized conditions.补充表1. 全混合日粮（Total Mixed Ration, TMR）的原料组成及化学成分（以干物质Dry Matter, DM为基准的百分比）。 补充表2. 提取变量（乙醇浓度、提取时间、重复次数）对提取率、总酚含量（Total Phenolic Content, TPC）及总黄酮含量（Total Flavonoid Content, TFC）影响的方差分析（Analysis of Variance, ANOVA）结果。 补充表3. 预测响应面二次模型的回归系数及回归参数的方差分析结果。 补充表4. 优化条件下提取率、总酚含量（TPC）及总黄酮含量（TFC）的预测值与实验值对比。