Raw data on the effect of resource availability and herbivory on the defence-growth-reproduction trade-off of a masting Mediterranean pine

[General methods] The full collection of six csv files contains all the raw data used in the paper “Resource availability and herbivory alter defence-growth-reproduction trade-offs in a masting Mediterranean pine”, which is now in its pre-print version. In this article we report the results of two parallel experiments we carried out to independently asses the effect of nutrient availability and different defence induction treatments on the three-way trade-off. Thirty six ramets were allocated to the fertilization experiment, with 12 ramets of each of three genotypes being randomly assigned to one of three blocks and one of two treatments (fertilization and control). Within each block, ramets were spatially grouped in two treatment plots. Each treatment by genotype combination comprises six ramets, two per block. The remaining 45 ramets were included in the defence induction experiment and randomly assigned to one of three blocks and one of five induction treatments. Within each block, ramets were spatially grouped in five treatment plots. Each treatment by genotype combination had three ramets, one per block. In the fertilization experiment we had only two treatments (fertilized vs control). Fertilization was accomplished by applying Fertimon Rojo (Soaga S.L. 2-10-16 NPK + 2.8% Mg) and a liquid 100-20-70 NPK fertilizer applied to the substrate at the base of the trunk of each tree (see Sampedro et al. 2011 for details on this fertilizer solution). The foliar fertilization continued until the end of the experiment. In january 2010, 125 additional grams of Osmocote (KB®, Scotts, NPK 17-09-11 + 2% MgO) were also added to each tree. Fertilization was maintained throughout the whole monitoring period (2010-2015). Five treatments were assigned to the herbivory simulation experiment (applied during the first two years, 2010-2011): • Needle clipping (repeated cutting of needles tips, around 1-2 cm). • Methyl jasmonate application (brushing a 25 mM solution on debarked areas of the trunk; see details in Vázquez-González et al. 2021). • Low intensity mechanical wounding of the trunk involved making three 5 mm circular holes in the bark and phloem. • High intensity mechanical wounding of the trunk, throug six 5 mm circular hole in the bark and phloem Ramets were monitored from 2010 to 2015. Below, we provide specific details on the methods associated to its data-set and the variables it includes.