Additional file 7 of Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors

Supplementary file 1. Raw spike counting and bar charts of ac4-enhanced activation from various transgenic flies. Control fly line genotypes: Oregon-C wild type, w;IR75d-Gal4;IR75dKO, w;UAS-IR75dHEK;IR75dKOand w;Bl/CyO;IR84a-Gal4KI. Fly-line genotypes expressing heterologous IRs: w;IR76a-Gal4;UAS-CpomIR41a1, w;IR75d-Gal4/UAS-DsuzIR75dHEKK;IR75dKO, w;UAS-DsuzIR64a;IR84a-Gal4KI, and w;UAS-CpomIR64a∆;IR84a-Gal4KI. Choice of p-values from a parametric or a non-parametric statistical analysis depended on tests for normal distribution. The magnitude of p-values has been distinguished as it follows: * p < 0.05; ** 0.001 < p < 0.01; *** p < 0.001; which is consistent with Figure 1. Note: colors have been used to distinguish genotypes and remark their significant effects based on colors used in Figure 1. For the tested ligands, blue names indicate ligands diluted in water and red ligands diluted in ethanol.