An inflamed human alveolar model for testing the efficiency of anti-inflammatory drugs in vitro

The data set accompanies the study where we developed an inflamed human alveolar epithelium model and to test the resolution<strong><em> </em></strong>lipopolysaccharide (LPS)-induced inflammation <em>in vitro</em> with a corticosteroid, methylprednisolone (MP). A specific focus of the study was in macrophage phenotype shifts in response to these stimuli. The data set includes: - Pro-inflammatory marker (interleukin (IL)-8, tumor necrosis factor α (TNFα), IL1β) secretion data, analysed via ELISA, and cell viability (analysed via lactate dehydrogenase assay) of both monocultures (human monocyte-derived macrophages) and of the multicellular human alveolar model, composed of macrophages, dendritic cells, and epithelial cells. - Barrier permeability data of the multicellular model, assessed via labeled-dextran permeability assay. All the above-stated data is joined in the file: DraslerB_Frontiers 2020_Inflammatory model. Sample codes are explained int he first tabs. - Pro-inflammatory marker gene expression data of the multicellular model, assessed via real time RT-qPCR. The data prepared for analysis and analysed is joined to the above-mentioned folder, whereas the direct PCR runs are joined in the file: DraslerB_Frontiers 2020_Inflammatory model_PCR runs. - Confocal laser scanning microscopy raw files (lsm) of the multicellular model can be opened with an open source software Fiji, based on ImageJ.