Genome wide analysis of the effect of p65 inhibition upon the epigenomic landscape in high passage growth arrested cells being withdrawn from rapamycin

BJ Fibroblasts were maintained in rapamycin and were passaged until reaching growth arrest at very high passages. These cells were then maintained in or withdrawn from rapamycin, during which time they were given either control treatments or the p65 inhibitor TPCA-1. BJ Fibroblasts were maintained in rapamycin and were passaged until reaching growth arrest at very high passages (>PD75). These cells were then maintained in or withdrawn from rapamycin for 2 weeks, during which time they were given either treated with control or the p65 inhibitor TPCA-1. Cells were then harvested for ChIP-seq of enhancer marking histone modification H3K27ac, or the transcription factor p65. ChIP-seq tag density was then compared between rapamycin withdrawal and maintained samples, as well as between treatment and control samples.