Comprehensive Evaluation of Human Brain Gene Expression Deconvolution Methods
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Expression profiling by high throughput sequencing.
5 RNA-seq samples generated from mixtures of cultured neuronal and astrocyte RNA (neuronal:astrocyte RNA ratios of 0:100,40:60,45:55,50:50, and 100:0). 10 RNA-seq samples of paired cytoplasmic and nuclear preparations from 5 bulk adult human brain tissue samples.
采用高通量测序技术开展基因表达谱分析。包含5个RNA测序（RNA-seq）样本，由体外培养的神经元与星形胶质细胞的RNA混合制备而成，神经元与星形胶质细胞RNA的混合比例分别为0:100、40:60、45:55、50:50及100:0。另包含10个RNA测序（RNA-seq）样本，取自5份成人全脑大块组织样本的配对细胞质与细胞核组分制备物。
