Additional file 2 of Screening of differentially expressed microRNAs and target genes in two potato varieties under nitrogen stress

Additional file 2: Figure 1. Analysis of variability among groups of differential miRNAsHN: excessive N application; LN: no N application; Y: Yanshu4; D: Atlantic;S: seedling stage; A: budding stage (A) LN_YLSvsLN_DLS (B) LN_YLAvsLN_DLA (C) HN_YLSvsHN_DLS. (D)HN_YLAvsHN_DLA (E) HN_DLSvsLN_DLS (F) HN_DLAvsLN_DLA. (G) HN_YLSvsLN_YLS (H)HN_YLAvsLN_YLA (I) LN_DLAvsLN_DLS. (J)HN_DLAvsHN_DLS (K)LN_YLAvsLN_YLS (L)HN_YLAvsHN_YLS.(M)LN_YRSvsLN_DRS (N)LN_YRAvsLN_DRA (O)HN_YRSvsHN_DRS. (P)HN_YRAvsHN_DRA (Q)HN_DRSvsLN_DRS(R)HN_DRAvsLN_DRA. (S)HN_YRSvsLN_YRS (T)HN_YRAvsLN_YRA (U)LN_DRAvsLN_DRS. (V)HN_DRAvsHN_DRS(W)LN_YRAvsLN_YRS (X)HN_YRAvsHN_YRS. Using the values of log2(fold change) as the horizontalcoordinate and -log10(p-value) as the vertical coordinate, volcanoplots were constructed for all miRNAs during differential expression analysis.The horizontal coordinate represents the fold change in the differentialexpression of miRNAs in different samples, and the vertical coordinaterepresents the statistical significance of the difference in the change inexpression levels of miRNAs. The red color represents up-regulatedsignificantly differentially expressed genes, the blue color representsdown-regulated significantly differentially expressed genes, and the grey dots representnon-significant differentially expressed genes. Figure 2. Comparison between groups of differential miRNAs. HN: excess N; LN:no N; Y: Yanshu4; D: Atlantic; S: seedling stage; A: budding stage. Thehorizontal coordinates indicate the data obtained after the comparison ofgroups, and the vertical coordinates indicate the number of up- anddown-regulated miRNAs. The red color represents up-regulated miRNAs, the bluecolor represents down-regulated miRNAs, and the numbers represent the number ofup- and down-regulated miRNAs. Figure 3. Results of clustering analysis ofdifferential miRNAs. HN: excess N; LN: no N; Y: Yanshu4; D: Atlantic; S: seedling;A: bud onset. (A)LN_YLSvsLN_DLS (B)LN_YLAvsLN_DLA (C)HN_YLSvsHN_DLS. (D)HN_YLAvsHN_DL.(E)HN_DLSvsLN_DLS (F)HN_DLAvsLN_DLA. (G)HN_YLSvsLN_YLS(H)HN_YLAvsLN_YLA (I)LN_DLAvsLN_DLS.(J)HN_DLAvsHN_DLS (K)LN_YLAvsLN_YLS (L)HN_YLAvsHN_YLS. (M)LN_YRSvsLN_DRS (N)LN_YRAvsLN_DRA(O)HN_YRSvsHN_DRS. (P)HN_YRAvsHN_DRA (Q)HN_DRSvsLN_DRS (R)HN_DRAvsLN_DRA. (S)HN_YRSvsLN_YR.(T)HN_YRAvsLN_YRA (U)LN_DRAvsLN_DRS. (V)HN_DRAvsHN_DRS (W)LN_YRAvsLN_YRS (X)HN_YRAvsHN_YRS