Diurnal transcriptome landscape of a multi-tissue response to time restricted feeding in mammals

Time-restricted feeding (TRF) is an emerging behavioral nutrition intervention that sustains a daily cycle of feeding and fasting. TRF in animals and humans has shown pleiotropic health benefits arising from multiple organ systems, yet the molecular basis of TRF is not well understood. We subjected mice to isocaloric ad libitum feeding (ALF) or TRF of Western diet, and examined gene expression changes in 22 different brain regions and peripheral organs collected every 2 hour over 24 hour. We discovered TRF profoundly impacts gene expression. Nearly 80% of genes show significant differential expression or rhythmicity under TRF in at least one tissue. Functional annotation of these changes highlight tissue and pathway specific impacts of TRF. These findings and resources will offer a framework for future mechanistic studies, and guide human TRE interventions for various disease conditions with or without pharmacotherapies. Overall design: Diurnal mRNA expression profiles from 22 tissues from mice subjected to ad-libitum feeding (ALF) or 9 hr time-restricted feeding (TRF) of Western diet. 1035 libraries were sequenced. Each library was sequenced 1 to 4 times (1677 total samples). The 'description' column includes the library names of the samples.