Dataset for: Highly Efficient One-Pot Bi-Enzymatic Cascade to 5-MeO-Tryptamine

This dataset contains the raw data and processed experimental data supporting the above publication. This study is focused on the development of a one-pot tandem two-enzyme cascade for the synthesis of 5-MeO-tryptamine from inexpensive substituted indole under mild and aqueous conditions. Bioinfomatic tools were applied to engineer the enzymes, EcTnaA and RgnTDC, and to give insights into their dynamics. The cascade could be operated with 5-MeO-indole as a solid suspension (beyond its solubility limit) in pure aqueous buffer, which improved decarboxylase performance, with a yield of 88% in just 4 h, and a space–time yield (STY) of 2.1 g L–1 h–1, corresponding to a 35-fold increase in STY compared to previously reported methods.