Behavioral data of empathy, anxiety and depression, and monoamine transmitters (DA/NE/5 -HT) content datasetin SD rats in CON, CON+CSCP, CUMS and CUMS+CSCP groups.

The data uploaded in this study is mainly divided into two parts.A behavioral experiment   Identification of partnership establishment: Using the time spent exploring on both sides of the familiar partner rat and the unfamiliar rat in the three-box preference experiment as an indicator, the SD rats' innate preference for unfamiliar mice (preferring to explore in the unfamiliar mouse side of the box) is used as an indicator. Familiarity was determined after two weeks of coexistence with partner mice. In Excel, the corresponding S% is the percentage of time on the side of the stranger mouse, and F% is the percentage of time on the side of the partner mouse. The test time is 8:30-16:00 during the day.    Detection of the level of empathic behavior: Using the social fear transfer model, the model is divided into two detection stages: receiving intermittent acoustic stimulation (FCbP) in the same room with the partner rat after receiving the combined stimulation of buzzing and electrical stimulation; Alone in a room, receiving intermittent acoustic stimulation (FET). In FCbP, social behavior with partner mice is mainly used as an indicator of emotional empathy, and in FET, freezing behavior is used as an indicator of cognitive empathy; here, social times% and social time%, and freeze times% and freeze time% are used as judgments index. Social behaviors and rigid behaviors are first recorded with a high-definition camera, and then marked and recorded by J-watcher behavior analysis software (marking records are available in excel), which is available for free at http://www.jwatcher.ucla.edu/ Download; In addition, the detection of USV or 22-kHz ultrasound emission can be carried out in the intermittent periods of the two stages to assist the differences in empathic behavior of the four groups of rats. The instrument used was an ultrasonic recorder (D1000x, Pettersson Eletronik AB, Sweden), and then Batsound 4.2 was used to count the duration and times of ultrasonic delivery (this software is sold with the recorder and cannot be downloaded for free). data is displayed.   Anxiety Behavior, Depression-Like Behavior, and Social Behavior Tests  3.1 Open field OFT experiment: test in a device developed by using SD rats to explore the contradiction between curiosity and anxiety in a new environment, in which the greater the activity in the central area, the lower the anxiety level of the subjects;  3.2 Elevated plus maze EPMT experiment: The device is divided into two closed arms and two open arms, forming a cross shape 1.5m above the ground. Among them, the longer the time spent in the closed arm, the less exploratory the subjects were and the higher the anxiety level.  3.3 Depression-like behavior: In addition to the weight test, the researchers used the lack of pleasure in depressed patients (the rate of preference for sugar water was reduced) and the increase in the degree of despair (in the tail suspension experiment, giving up struggling, and the length of the immobility time) was used for detection;  3.4 Using the sociality and social preference in the three-box experiment to evaluate the social cognitive ability of the four groups of rats. First, in the first stage of sociality, normal mice prefer strange mice to empty boxes, so the difference in the length and frequency of sniffing between strange mice and boxes is used to judge whether sociality is normal; Put another stranger rat 2 in the box, and use the duration and times of sniffing on stranger mouse 1 and stranger mouse 2 to judge whether their social cognition is normal; this experiment was also analyzed and recorded by J watcher software.2. High performance liquid phase experiment   In this experiment, samples were firstly collected from four groups of animals in low temperature environment, which are BLA/HiP/mPFC three brain regions strongly related to anxiety, depression and empathy. Then carry out cracking and sample preparation; finally, the content of DA/NE/5-HT was detected in the U3000 high performance liquid chromatograph of Thermo Fisher (Waltham, Massachusetts, USA). The peak time of NE was determined, and the regression curve of peak area and concentration was constructed for subsequent conversion of brain tissue content. After that, the corresponding samples of each tissue are numbered and put into the instrument for detection according to the set procedure. The exported files were converted to PDF files using Chromeleon 7.0 for data analysis. Finally, use the regression curve of each monoamine transmitter to convert the area under the peak corresponding to each sample to the concentration, and then complete.