Quantification of the quinone pool of suppressor strains.
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aFlhB proteins were expressed from plasmid vector.bCultures were harvested at mid-exponential phase, OD600 0.6.cStandard deviations are shown for measurements performed in triplicate.dN.D., None detected. Ubiquinone-8 was not detected in cells bearing a ubiE(G12V) mutation, however, 2-octaprenyl-6-methoxy-1,4-benzoquinone (480±150 ng/mg of cell protein) was detected.
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2015-12-02
