Developmental exposures to three mammalian teratogens produces dysmorphic phenotypes in adult Caenorhabditis elegans.

Adult morphology was assessed at sublethal concentrations in C. elegans using three mammalian teratogens, 5-fluorouracil (5FU), hydroxyurea (HU), or ribavirin (RV). C. elegans develop from egg to adult with internal eggs in about 70h at 20°C in C. elegans Habitation Medium (CeHM). Cohorts were exposed from first feeding after hatching for either 24h (early-only) or continuously for four days. Significant increases in the percentage of prolapsed individuals were seen on the first day of adulthood in both continuous and early-only 5FU and HU exposure groups. In contrast, continuous RV exposures induced gonad arm and vulval anomalies that were largely but not completely reversed in the early-only exposure groups. Reductions in reproductive output with continuous exposures recovered, though not fully, with early-only exposures for all three chemicals. To our knowledge, this is the first systematic evaluation of C. elegans morphology changes induced by mammalian teratogens. These data sugges..., The C. elegans wild-type N2 strain was obtained from the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Continuously well-fed cultures were maintained and exposed in CeHM as previously described (DOI: 10.1002/jat.4525). Sublethal concentrations that allowed for at least semi-synchronous developmental timing within exposed cohorts were previously determined (DOI: 10.1016/j.taap.2024.117108). For continuously exposed C. elegans, synchronized newly hatched cohorts were counted and diluted to approximately 1,000 first larval stage (L1) worms per mL CeHM, then 900 µL worms in CeHM were transferred to 12-well plates and 100 µL of 10x aqueous dosing solution was added per well within 30 min post-first-feeding after hatching. Plates were maintained in 20°C incubators for four days. For early-only exposures, the following alterations were made: after 24.5h +/-30min exposures, C. elegans cohorts were quickly washed first..., # Developmental exposures to three mammalian teratogens produces dysmorphic phenotypes in adult Caenorhabditis elegans

Dataset DOI: [10.5061/dryad.j3tx95xs9](10.5061/dryad.j3tx95xs9)

## Description of the data and file structure

As required by Dryad, this image data set is submitted to the public domain without restrictions or conditions for reuse, modification, or redistribution.

5-fluorouracil (5FU), hydroxyurea (HU), and ribavirin (RV) are mammalian teratogens that also cause growth retardation in mammals and toxicological model organisms, including *C. elegans*. The effects of 5FU, HU, and RV on *C. elegans* adult morphology were evaluated after continuous and 24h (early-only) exposures beginning from first feeding after hatching. 

Data: the images are .jpg files, organized in folders by chemical, experiment number, exposure duration, and exposure concentration.

Data description:* C. elegans* cohorts were exposed to sublethal concentrations of three mammalian teratogens from f...,