Data underlying the research on particle manipulation using hydrodynamic forcing. This dataset consist of the experimental data of particle interchanging its position in a Hele-Shaw cell.

The reseach objective is to present a microfluidic approach to achieve the dynamic control of particle pathlines within a flow through microfluidic device. Our approach combines three key aspects: the design of a flow-through microfluidic flow cell with the ability to manipulate the streamlines of the flow, an optimization procedure to find a priori optimal particle path-lines, and a Proportion-Integral-Derivative-based (PID) feedback controller to provide real time control over the particle manipulations. The experimental raw images were recorded with a sCMOS camera (PCO) with a pixel pitch of 6.5 μm. The camera was mounted on a microscope (Nikon Eclipse Ti) with a 1x objective. The<br> acquisition frequency was 5 Hz corresponding to an average in-plane displacement of 4-6 pixels between two consecutive recordings. The zip file contains the raw images and the MATLAB script used to do an experiment of two particles interchanging their positions by only using the hydrodynamic forcing in a Hele-Shaw cell.本研究的目标是提出一种微流控方法，以实现微流控装置内流场中粒子迹线的动态调控。本方法结合了三个核心环节：一是设计可操控流场流线的流通式微流控流池，二是用于求解先验最优粒子迹线的优化流程，三是基于比例-积分-微分（Proportion-Integral-Derivative, PID）的反馈控制器，以实现对粒子操控的实时调控。实验原始图像由sCMOS相机（PCO）采集，该相机的像素间距为6.5 μm。相机搭载于尼康（Nikon）Eclipse Ti显微镜，搭配1×物镜。采集频率为5 Hz，对应两次连续采集之间的平面内平均位移为4至6个像素。该压缩包包含原始图像，以及用于实现赫勒-肖（Hele-Shaw）流池内仅通过流体动力驱动完成两个粒子位置互换实验的MATLAB脚本。