Norovirus Files

Design of multi-epitope based vaccines for Norovirus. These Norovirus data were subsequently screened for complete structural protein selection via VaxiJen v2.0 to predict antigenic proteins. Depending on T-cell and B-cell epitope prediction, these antigenic peptides are further screened based on major histocompatibility complex (MHC) class I binding, proteasomal C terminal cleavage, and TAP transport efficiency using NetCTL1.2 Server. The promising epitopes were then selected, based on toxicity (ToxinPred), antigenicity (VaxiJen v2.0), allergenicity (AllerTop v.2.0), conservancy (ECA IEDB Suite), and higher immunogenicity (C1I IEDB Suite) filtration. These epitopes should then be identified by (MHC) I alleles and MHC II alleles which are present in the endoplasmic reticulum, so that it can bind to them, and can be brought to the surface of the cell, where a T-cell and a B-cell can identify to produce an antibody. In order to bind these epitopes with alleles, the 3D structures of epitopes were generated and the corresponding allele was retrieved from RCSB to perform docking. The stability and conformational changes of these two bindings along with their dynamic interactions are then analyzed, through molecular dynamics.