The presence of FLT3-ITD and mutated NPM1 in ALDH^bright and ALDH^low compartments in CD34-negative AML.

Detection of molecular aberrancies in CD34+CD38– ALDHbright and ALDHlow compartments of CD34-negative AML. The molecular nature of the CD34+CD38-ALDHbright and CD34– compartments in CD34-negative AML cases. Detection of FLT3-ITD and mutated NPM1 was performed in the various cell fractions. The FLT3-ITD percentage is determined in the total leukemic blast population (data not shown), the CD34+CD38-ALDHbright and CD34– compartments. The NPM1 mutation analysis is not quantative.