BM-1 (2018-4) Bone Marrow Microphysiological System Independent Measures Power Calculation

The purpose was to test the power calculation of n=6 in independent measures of cells from the BM MPS system using compound AZ1. This tested if an n=6: gives less variable data and increases confidence in responses seen, allows detection of toxicity at lower doses of compound AZ1 than previously tested, and allows confident detection of recovery.
Conclusion:
The power calculation of n=6 is appropriate for this independent measures design. However, the next experiment should investigate a repeated measures design to reduce variability chip to chip.
Notes:
In this independent measures study design, the cells were sampled from the scaffold well of the chip, meaning the chip was terminally harvested and the next timepoint's samples were from different chips. These samples are marked with an 'S'. Cells were also samples from the media well of the chip, labelled as 'F' for floating. To standardize voltage settings across timepoints, single color controls were included each day to calculate compensation. Each dosed group had 6 replicates.