Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke

Neuroinflammation is a hallmark of ischemic stroke, a leading cause of death and long-term disability. However, the exact cellular signaling pathways initiating and propagating neuroinflammation after stroke are unresolved and will be critical for developing immunomodulatory stroke therapies. We used the RiboTag technique to obtain astrocyte and microglia-derived mRNA transcripts in a hyperacute (4 hours) and acute (3 days) period after stroke, as these two cell types are key modulators of neuroinflammation. We report here an in-depth characterization of the microglia and astrocyte translatomes after stroke.