APJCell-Supplementary Figure 1. PRLR immunofluorescence and signaling pathway activation by PRL in small intestine and enterocytes of Prlr +/+ and Prlr−/− mice.

PRLR immunofluorescence and signaling pathway activation by PRL in small intestine and enterocytes of Prlr +/+ and Prlr−/− mice. A) Representative immunofluorescence images of the proximal small intestine from lactating Prlr +/+ and Prlr −/− mice at postnatal days 14 (PD14) and 21 (PD21) stained for the PRL receptor (PRLR). n = 3 mice per group. B) Epithelial cells isolated from intestinal crypts of Prlr+/+ and Prlr−/− mice at postnatal days 10-12 immunostained for PRLR and villin (n=3). C) Representative immunoblots of intestinal epithelial cell cultures from Prlr +/+ and Prlr −/− mice treated with PRL for 30 min. The graphs showing densitometric analysis of immunoblots for AKT and ERK1/2. Data shown are from three independent experiments (two wells per condition per experiment), using 1–4 mice per experiment. Data represent triplicate determinations ± SEM; *p < 0.05 vs. control, Student’s t-test.