Analysis of S. pneumoniae DNA sequence polymorphism using DnaSP program†.

a: Sequences were concatenated in the order 16S rRNA, hemolysin-related protein, putative hemolysin A, hemolysin, DNA-directed RNA polymerase – beta subunit, cell wall surface anchor family protein 1, cell wall surface anchor family protein 2, cell wall surface anchor family protein 3, antigen -cell wall surface anchor family protein, pneumococcal surface protein A, putative pneumococcal surface protein.b: Order of sequence concatenation for 5 conserved genes was 16S rRNA, hemolysin-related protein, putative hemolysin A, hemolysin, DNA-directed RNA polymerase – beta subunit for the analysis.c: Six variable gene sequences were concatenated in the order cell wall surface anchor family protein 1, cell wall surface anchor family protein 2, cell wall surface anchor family protein 3, antigen -cell wall surface anchor family protein, pneumococcal surface protein A, putative pneumococcal surface protein. for the analysis.*: Data normalized for net number of sites and expressed per 1000 bases. Raw values are shown in the parentheses.†: Data generated using DnaSP program, version 5.1.