Proteomic analysis of WT macrophages that have phagocytosed SILAC-bacteria

Macrophage are phagocytic cells that reprogram their cellular metabolism depending on the nature of innate immune receptors engaged upon microbial encounter. Yet, whether engulfed microbes constitute a source of nutrients for macrophages and how this contribute at establishing a specific metabolic reprogramming is poorly defined. Our study aims at understanding how microbial-derived amino acids feed macrophage metabolism by tracing labelled microbial amino acid in macrophage proteins. Here we analyzed the incorporation of labelled amino acids derived from dead bacteria into macrophage proteins at different time points.