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Raw, trimmed, and mapped, small RNA seq reads

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Figshare2023-01-28 更新2026-04-08 收录
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https://figshare.com/articles/dataset/Raw_trimmed_and_mapped_small_RNA_seq_reads/21922752/1
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Raw and trimmed small RNA seq reads obtained from <em>Psylliodes chrysocephala </em>treated with double-stranded (ds) RNAs (dsSec23, dsVatpG, or dsGPF). The".bam" files are the output of Bowtie2, representing the coverage of reads perfectly mapping onto the respective dsRNA treatment. "_reads" files are the raw reads obtained by sequencing on a DNBSEQ-G400 platform and the "_trimmed.fq.gz" files represent the reads cleaned from adaptor sequences as well as reads having quality scores below 30 and lengths below 18 nt and above 25. The bam files could be investigated by, for instance, R package "viRome" which can output the coverages per position on the respective dsRNA sequence or directly using a software such as Tablet for which the dsRNA index files are required and also provided here. Of note the dsRNA sequence in the index file dsVatpG_index is antisense of its target gene whereas for dsSec23_index it is in sense direction. Another important note regarding the directionality is that all the raw reads are <br>
提供机构:
CEDDEN, Doga
创建时间:
2023-01-20
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